Mutagenic and Antimutagenic Effects of the Traditional Herb Used for Treating Erectile Dysfunction, Butea superba Roxb.

Cherdshewasart W, Sutjit W, Pulcharoen K, Chulasiri M.

Abstract

Butea superba is a traditional tuberous Thai plant enriched with flavonoids that is used for treating erectile dysfunction. We investigated the mutagenic and antimutagenic potentials of a B. superba extract by using the pre-incubation method of the Ames test. Salmonella typhimurium strains TA 98 and TA 100 were applied as the tester strains in the presence and absence of an S9 mixture. Prior to the mutagenic and antimutagenic tests, the survival of the tester strains was measured by treating with the B. superba extract. The results show that the B. superba extract exhibited dose-dependent cytotoxic effects. Data from the Ames test revealed that the B. superba extract to be non-mutagenic in the presence and absence of the S9 mixture. In contrast, the B. superba extract showed antimutagenic potential towards either or both of the tested mutagens: 2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide (AF-2) and benzo(a)pyrene (B(a)P) in the respective presence and absence of the S9 mixture, respectively. The plant antimutagenic activity was confirmed by a rec assay. A further study by micronucleus test demonstrated that the B. superba extract at the maximum loading volume could induce acute micronucleus formation in the tested animals. The in vitro mutagenic and antimutagenic assays confirmed the safe consumption of B. superba products at low dose (not more than 781.25 µg/ml of the plant extract), but the in vivo genotoxic assay demonstrated the unsafe consumption at a high dose (300 mg/kg of the BW plant extract or 16 g/kg of the BW plant powder).

References:

Adamopoulos, D. A., Pappa, A., Nicopoulou, S., Andreou, S., Karamertzans, M., & Michopoulos, J. (1996). Seminal volume and total sperm number: Trends in men attending subfertility clinics in the greater Athens area during the period 1977-1993. Human Reproduction, 11, 1936-1941.

Check, J. H., Bollendorf, A., Lee, M. A., Nazari, A., & Noowroozik, K. (1990). Correlation of computerized semen analysis with successful fertilization of oocytes in an in vitro fertilization program. Archieves of Andrology,24, 229-234.

Donnelly, E. T., Lewis, S. E. M., Thompson, W., & Chakravarthy, U. (1997). Sperm nitric oxide and motility: The effects of nitric oxide synthase stimulation and inhibition. Molecular Human Reproduction, 3, 755-762.

The use of Butea superba (Roxb.) compared to sildenafil for treating erectile dysfunction BJU Int. 2010;105:225–8

Borrelli, F; Colalto, C; Delfino, DV; Iriti, M; Izzo, AA. (2018) Herbal Dietary Supplements for Erectile Dysfunction: A Systematic Review and Meta-Analysis. Drugs, Volume 78, pages 643–673.

Mikosch, M; Kumari, N; Sharma, T; Sharma, V; Gessler, A; Fischer-Schliebs, E; Lüttge, U. (2012) Plasticity of photosynthetic performance of the Indian tree Butea monosperma TAUB. at three sites with different microclimates. Photosynthesis Research, Volume 113, pages 287–295, https://link.springer.com/article/
10.1007/s11120-012-9770-5

Chauhan, NS; Sharma, V; Dixit, VK; Thakur, M. (2014) A Review on Plants Used for Improvement of Sexual Performance and Virility. BioMed Research International, https://doi.org/10.1155/2014/868062